Real Time PCR Master Mixes
G-Biosciences Probe-Based One Step RT-qPCR 2X Master Mix
Probe-based one step RT-qPCR 2X master mix is used for gene expression analysis, genotyping and mutation detection, pathogen detection, viral detection, absolute quantification. It has enhanced efficiency, specificity, sensitivity and compatible with nearly all real time PCR instruments.
G-Biosciences SYBR™ Green-Based qPCR 2X Master Mix
SYBR™ Green based qPCR 2X master mix is used for gene expression analysis, genotyping and mutation detection, pathogen detection, viral detection, absolute quantification. It has enhanced efficiency, specificity, sensitivity and compatible with nearly all real time PCR instruments.
LIFE TECHNOLOGIES TAQMAN FAST ADVANCED NO UNG MM
A44360 TAQMAN FAST ADVANCED NO UNG MM
LIFE TECHNOLOGIES TAQMAN FAST ADVANCED NO UNG MM
A44359 TAQMAN FAST ADVANCED NO UNG MM
Bio Rad Laboratories SsoAdvanced Universal SYBR Green Supermix, 500 x 20 uL rxns, 5 mL
5 ml (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR Green I, ROX Normalization Dyes, for 500 x 20 uL reactions.
New England Biolabs, Inc. Gibson Assembly® Master Mix – 10 reactions
Gibson Assembly allows for successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility. Gibson Assembly efficiently joins multiple overlapping DNA fragments in a single-tube isothermal reaction. The Gibson Assembly Master Mix includes three different enzymatic activities that perform in a single buffer: The exonuclease creates single-stranded 3 overhangs that facilitate the annealing of fragments that share complementarity at one end (overlap region).
- Assembly and transformation in just under two hours
- Flexible sequence design (scar-less cloning)
- No PCR clean-up step required
- High transformation efficiencies for inserts up to 20 kb
- Easily adapted for multiple DNA manipulations, including site-directed mutagenesis
LGC GENOMICS LLC (Lucigen) EconoTaq PLUS GREEN 2X Master Mix, 500 RxnsDependable Taq polymerase performance.Choice of reaction buffers, with or without MgCl2.Non-proofreading Polymerase
EconoTaq PLUS GREEN & EconoTaq PLUS 2X Master Mixes offer Performance, Convenience, Reliability, and Value for Routine PCR. More effective than other Taq master mixes. EconoTaq PLUS and PLUS GREEN 2X Master Mixes contain a proprietary PCR enhancer, so they can successfully amplify templates that fail to amplify with other PCR master mixes. High density reaction buffer enables direct gel loading following PCR. Excellent results in routine PCR. EconoTaq PLUS GREEN & EconoTaq PLUS contain high purity, high activity EconoTaq DNA Polymerase for reliable amplification of templates up to 5 kb. Amplify challenging GC-rich templates. These 2X Master Mixes can be cycled up to 98C to amplify challenging GC-rich templates others cannot. Convenient tracking dyes in EconoTaq PLUS GREEN. On a 1% agarose gel, the blue dye migrates at the same rate as a 5 kb DNA fragment, and the yellow dye migrates at 75 bp.
New England Biolabs, Inc. Gibson Assembly® Master Mix – 50 reactions
Gibson Assembly allows for successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility. Gibson Assembly efficiently joins multiple overlapping DNA fragments in a single-tube isothermal reaction. The Gibson Assembly Master Mix includes three different enzymatic activities that perform in a single buffer: The exonuclease creates single-stranded 3 overhangs that facilitate the annealing of fragments that share complementarity at one end (overlap region).
- Assembly and transformation in just under two hours
- Flexible sequence design (scar-less cloning)
- No PCR clean-up step required
- High transformation efficiencies for inserts up to 20 kb
- Easily adapted for multiple DNA manipulations, including site-directed mutagenesis
New England Biolabs, Inc. Luna Universal qPCR Master Mix – 200 rxn (2 x 1 ml)
The NEB Luna Universal qPCR Master Mix is an optimized 2X reaction mix for real-time qPCR detection and quantitation of target DNA sequences using the SYBR/FAM channel of most real-time qPCR instruments. It contains Hot Start Taq DNA Polymerase and has been formulated with a unique passive reference dye that is compatible across a variety of instrument platforms (including those that require a high or low ROX reference signal). It also features dUTP for carryover prevention and a non-fluorescent, visible dye to monitor reaction setup. This dye does not spectrally overlap with fluorescent dyes used for qPCR and will not interfere with real-time detection. The master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA except primers and DNA template. Genomic DNA or cDNA of interest can be quantitated with Luna qPCR, and existing as well as commercial qPCR assay primer sequences can be used.
ABclonal Technology 2X Universal SYBR Green Fast qPCR Mix, 25 mL, SYBR Green I, ABclonal Hot Start Taq DNA Polymerase, Universal ROX Dye
The ABclonal 2X Universal SYBR Green Fast qPCR Mix is a highly efficient solution tailored for sensitive real-time quantitative PCR applications. This ready-to-use mix combines advanced components including a hot start polymerase, optimized buffers, and a proprietary dye, ensuring accurate results across a variety of qPCR instruments. Its formulation minimizes non-specific amplification and enhances specificity across diverse samples.
- 2X concentrated format
- Compatible with all qPCR instruments
- Hot start capability for improved specificity
- Includes SYBR Green I and universal ROX dye
- Demonstrates high reaction efficiency (99%)
- Features excellent freeze-thaw stability
- Recommended for target lengths of 70-200 bp
- Storage temperature of -20℃
- Used for research purposes only
- Protect from light during storage
Meridian Life Science SensiFAST SYBR No-ROX Kit, 500 x 20 uL Reactions
Outstanding assay reproducibility, sensitivity and robustness from both DNA and RNA templates, under fast thermal cycling conditions.
Biotium Fast Probe Master Mix (500 rxn)
Fast Probe Master Mix is formulated for conducting real-time PCR using oligo-based probes such as TaqMan or MGB probes. The master mix contains buffer, dNTPs, MgCl2 and our superior hotstart enzyme Cheetah™ Taq. Cheetah™ Taq is a chemically modified hotstart Taq which can be fully activated in 2 minutes, thus permitting fast PCR. We offer two formats of probe master mix, without ROX and with ROX.For sensitive, affordable qPCR reactions we now recommend our new and improved Forget-Me-Not™ Universal Probe PCR Master Mix.TaqMan is a registered trademark of Roche Diagnostics.